(379) Ploidy Determinationin Vitis rotundifolia using Flow Cytometry
نویسندگان
چکیده
منابع مشابه
A survey of apomixis and ploidy levels among Poa L. (Poaceae) using flow cytometry
Poa is a complex genus taxonomically and genetically. As a result, relatively little information exists for this economically important genus with regards to reproductive mode and variability in chromosome number. We examined apomixis frequency and ploidy levels in 83 Poa accessions representing 33 species from the National Plant Germplasm System using fl ow cytometric techniques. In reproducti...
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Factors influencing Agrobacterium-mediated transformation of 15 Vitis rotundifolia, Vitis rupestris, Vitis vinifera and Vitis hybrids were explored. A green fluorescent protein/neomycin phosphotransferase II (GFP/NPT II) fusion gene was used to measure transient and stable transgene expression levels. Embryogenic cell cultures at different developmental stages were co-cultivated with Agrobacter...
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flow cytometry (fcm) was used to determine the ploidy levels of six different populationsof agropyron cristatum (l.) gaertn. (poaceae) in iran, using a diploid cultivated barley (hordeumvulgare l.) as an internal reference. according to flow cytometric data, tetraploid (2n = ± 28) andhexaploid (2n = ± 42) levels were detected among the examined populations and these results weresupported by chr...
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A flow cytometric method for the quantitation of reticulocytes was refined for routine laboratory use. Blood (2 microliters) is added to 2 ml of 0.4 microM thiazole orange in phosphate buffered saline, incubated at room temperature for 90 minutes, and analysed on a Coulter EPICS Profile flow cytometer, with gating for red cells on the basis of forward and right angled light scatter. Blood (2 mi...
متن کاملFlow cytometry (ploidy determination, cell cycle analysis, DNA content per nucleus)
During recent years, flow cytometry has established itself as a useful, quick novel method to determine efficiently, reproducibly and at a reduced cost per sample the relative nuclear DNA content and ploidy level of a large number of species (plants and animals), that has also been used to sort cells with different traits from a mixed cell population (e.g. as when separating heterokaryons from ...
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ژورنال
عنوان ژورنال: HortScience
سال: 2005
ISSN: 0018-5345,2327-9834
DOI: 10.21273/hortsci.40.4.1068c